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The 'mitoflash' probe cpYFP does not respond to superoxide

Schwarzländer, M; Wagner, S; Ermakova, YG; Belousov, VV; Radi, R; Beckman, JS; Buettner, GR; ... Murphy, MP; + view all (2014) The 'mitoflash' probe cpYFP does not respond to superoxide. Nature , 514 (7523) E12-E14. 10.1038/nature13858. Green open access

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Abstract

Ageing and lifespan of organisms are determined by complicated interactions between their genetics and the environment, but the cellular mechanisms remain controversial; several studies suggest that cellular energy metabolism and free radical dynamics affect lifespan, implicating mitochondrial function. Recently, Shen et al.1 provided apparent mechanistic insight by reporting that mitochondrial oscillations of ‘free radical production’, called ‘mitoflashes’, in the pharynx of three-day old Caenorhabditis elegans correlated inversely with lifespan. The interpretation of mitoflashes as ‘bursts of superoxide radicals’ assumes that circularly permuted yellow fluorescent protein (cpYFP) is a reliable indicator of mitochondrial superoxide2, but this interpretation has been criticized because experiments and theoretical considerations both show that changes in cpYFP fluorescence are due to alterations in pH, not superoxide3, 4, 5, 6, 7. Here we show that purified cpYFP is completely unresponsive to superoxide, and that mitoflashes do not reflect superoxide generation or provide a link between mitochondrial free radical dynamics and lifespan. There is a Reply to this Brief Communication Arising by Cheng, H. et al. Nature 514, http://dx.doi.org/10.1038/nature13859 (2014).

Type: Article
Title: The 'mitoflash' probe cpYFP does not respond to superoxide
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/nature13858
Publisher version: http://dx.doi.org/10.1038/nature13858
Language: English
Additional information: Copyright © 2014 Macmillan Publishers Limited. All rights reserved.
Keywords: Animals, Caenorhabditis elegans, Longevity, Male, Mitochondria, Superoxides
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/1454231
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