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Morphologic effect of dimethyl sulfoxide on the blood-brain barrier.

Broadwell, RD; Salcman, M; Kaplan, RS; (1982) Morphologic effect of dimethyl sulfoxide on the blood-brain barrier. Science , 217 (4555) pp. 164-166. 10.1126/science.7089551.

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Abstract

Dimethyl sulfoxide (DMSO) opens the blood-brain barrier of mice to the enzymatic tracer horseradish peroxidase. A single injection of horseradish peroxidase in 10 to 15 percent DMSO into the tail vein along with 10 to 15 percent DMSO delivered intraperitoneally allowed horseradish peroxidase to fill the extracellular clefts throughout the brain within 2 hours. In the absence of DMSO, peroxidase failed to enter brain parenchyma except through the circumventricular organs. Opening of the blood-brain barrier by DMSO is reversible. Dimethyl sulfoxide stimulated the pinocytosis of horseradish peroxidase by the cerebral endothelium; the peroxidase was then directed to lysosomal dense bodies for degradation. Vesicular transport of horseradish peroxidase from the luminal to the abluminal wall of the endothelial cell was not observed. Dimethyl sulfoxide did not alter the morphology of endothelial cells or brain parenchyma.

Type: Article
Title: Morphologic effect of dimethyl sulfoxide on the blood-brain barrier.
Location: United States
DOI: 10.1126/science.7089551
Keywords: Animals, Blood-Brain Barrier, Brain Chemistry, Dimethyl Sulfoxide, Endothelium, Extracellular Space, Female, Horseradish Peroxidase, Injections, Intraperitoneal, Injections, Intravenous, Mice, Pinocytosis
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst of Clinical Trials and Methodology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst of Clinical Trials and Methodology > MRC Clinical Trials Unit at UCL
URI: http://discovery.ucl.ac.uk/id/eprint/1448623
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