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A Spindle Assembly Checkpoint Protein Functions in Prophase I Arrest and Prometaphase Progression

Homer, H; Gui, L; Carroll, J; (2009) A Spindle Assembly Checkpoint Protein Functions in Prophase I Arrest and Prometaphase Progression. SCIENCE , 326 (5955) 991 - 994. 10.1126/science.1175326.

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Abstract

Two critical stages of mammalian oocyte regulation are prophase I arrest, which is important for sustaining the oocyte pool, and the progression through meiosis I (MI) to produce fertilizable eggs. We have found that the spindle assembly checkpoint protein BubR1 regulates both stages in mouse oocytes. We show that oocytes depleted of BubR1 cannot sustain prophase I arrest and readily undergo germinal vesicle breakdown, a marker for reentry into MI. BubR1-depleted oocytes then arrest before completing MI, marked by failure of polar body extrusion. Both meiotic defects in BubR1-depleted oocytes are due to reduced activity of the master regulator known as the anaphase-promoting complex (APC), brought about through diminished levels of the APC coactivator Cdh1.

Type:Article
Title:A Spindle Assembly Checkpoint Protein Functions in Prophase I Arrest and Prometaphase Progression
DOI:10.1126/science.1175326
Keywords:MOUSE OOCYTES, PREVENTS ANEUPLOIDY, APC(CDH1) ACTIVITY, CYCLIN-B, M-PHASE, SECURIN, MAD2, PROTEOLYSIS, COMPLEX, ENTRY
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology
UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Women's Health > Reproductive Medicine

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