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Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection.

Brown, JS; Ogunniyi, AD; Woodrow, MC; Holden, DW; Paton, JC; (2001) Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection. Infect Immun , 69 (11) pp. 6702-6706. 10.1128/IAI.69.11.6702-6706.2001.

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Abstract

There has been considerable recent research into protein based Streptococcus pneumoniae vaccines as alternatives to the existing capsular antigen vaccines. PiuA and PiaA (formerly Pit1A and Pit2A) are recently identified lipoprotein components of S. pneumoniae iron uptake ABC transporters which are required for full virulence and are likely to be expressed on the surface of the bacterial cell membrane. We investigated the efficacy of recombinant PiuA and PiaA proteins at eliciting protective immunity in mice against systemic infection with S. pneumoniae. Both recombinant PiuA and PiaA generated antibody responses that cross-reacted with each other but not with pneumolysin and reacted with identical proteins from nine different S. pneumoniae serotypes. Mice immunized with recombinant PiuA and PiaA were protected against systemic challenge to a degree similar to those immunized with an existing protein vaccine candidate, PdB (a genetically modified pneumolysin toxoid). Immunization with a combination of both PiuA and PiaA resulted in additive protection and was highly protective against systemic infection with S. pneumoniae. PiuA and PiaA are therefore promising additional candidates for a novel S. pneumoniae vaccine using protein antigens.

Type: Article
Title: Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection.
Location: United States
DOI: 10.1128/IAI.69.11.6702-6706.2001
Keywords: ATP-Binding Cassette Transporters, Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Proteins, Electrophoresis, Polyacrylamide Gel, Histidine, Immunization, Passive, Iron, Lipoproteins, Mice, Mice, Inbred BALB C, Pneumococcal Infections, Pneumococcal Vaccines, Recombinant Fusion Proteins, Sodium Dodecyl Sulfate, Streptococcus pneumoniae, Vaccination, Vaccines, Synthetic
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/144421
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