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Contrasting effects on HIF-1 alpha regulation by disease-causing pVHL mutations correlate with patterns of tumourigenesis in von Hippel-Lindau disease

Clifford, SC; Cockman, ME; Smallwood, AC; Mole, DR; Woodward, ER; Maxwell, PH; ... Maher, ER; + view all (2001) Contrasting effects on HIF-1 alpha regulation by disease-causing pVHL mutations correlate with patterns of tumourigenesis in von Hippel-Lindau disease. HUM MOL GENET , 10 (10) 1029 - 1038.

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Abstract

The von Hippel-Lindau tumour suppressor gene product (pVHL) associates with the elongin B and C and Cul2 proteins to form a ubiquitin-ligase complex (VCBC), To date, the only VCBC substrates identified are the hypoxia-inducible factor alpha subunits (HIF-1 alpha and HIF-2 alpha), However, pVHL is thought to have multiple functions and the significance of HIF-1 alpha and HIF-2 alpha regulation for tumour suppressor activity has not been defined. VHL disease is characterized by distinct clinical subtypes, Thus haemangioblastomas (HABs) and renal cell carcinoma (RCC) but not phaeochromocytoma (PHE) occur in type 1 VHL disease. Type 2 subtypes are characterized by PHE susceptibility but differ with respect to additional tumours (type 2A, PHE+HAB but not RCC; type 2B, PHE+ HAB+RCC; type 2C, PHE only). We investigated in detail the effect of 13 naturally occurring VHL mutations (11 missense), representing each phenotypic subclass, on HIF-alpha subunit regulation. Consistent effects on pVHL function were observed for all mutations within each subclass. Mutations associated with the PHE-only phenotype (type 2C) promoted HIF-alpha ubiquitylation in vitro and demonstrated wildtype binding patterns with pVHL interacting proteins, suggesting that loss of other pVHL functions are necessary for PHE susceptibility. Mutations causing HAB susceptibility (types 1, 2A and 28) demonstrated variable effects on HIF-a subunit and elongin binding, but all resulted in defective HIF-alpha( regulation and loss of p220 (fibronectin) binding. All RCC-associated mutations caused complete HIF-alpha dysregulation and loss of p220 (fibronectin) binding. Our findings are consistent with impaired ability to degrade HIF-alpha subunit being required for HAB development and RCC susceptibility.

Type:Article
Title:Contrasting effects on HIF-1 alpha regulation by disease-causing pVHL mutations correlate with patterns of tumourigenesis in von Hippel-Lindau disease
Keywords:TUMOR-SUPPRESSOR GENE, ENDOTHELIAL GROWTH-FACTOR, RENAL-CARCINOMA CELLS, FACTOR MESSENGER-RNA, GERMLINE MUTATIONS, VHL GENE, PROTEIN, HYPOXIA, BINDING, PRODUCT
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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