Ratnaraj, N; Patsalos, PN; (1998) A high-performance liquid chromatography micromethod for the simultaneous determination of vigabatrin and gabapentin in serum. THER DRUG MONIT , 20 (4) 430 - 434.
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A gradient high-performance liquid chromatography micromethod is described for the simultaneous quantitation of vigabatrin and gabapentin in human serum. Chromatography was performed using a 125- x 3-mm ID Hypersil BDS C-18 column with a 3-mu m mini-bore, eluted with a gradient system comprised of phosphate buffer (pH 6.5)-acetonitrile-methanol-water at a flow rate of 0.45 ml/minute. The column eluent was monitored on a fluorescence detector using excitation and emission wavelengths of 340 and 440 nm, respectively. The lower limit of quantitation for vigabatrin and for gabapentin was 5 mu mol/l, and the within batch and between-batch coefficients of variation were <5%. No interference from commonly prescribed antiepileptic drugs (carbamazepine and its metabolite carbamazepine epoxide, oxcarbazepine and its metabolite 10-hydroxyicarbazepine, ethosuximide, lamotrigine, phenobarbitone, phenytoin, primidone, and valproic acid) was observed; thus, the method can be used to monitor vigabatrin and gabapentin in patients on polytherapy antiepileptic drug regimens.
|Title:||A high-performance liquid chromatography micromethod for the simultaneous determination of vigabatrin and gabapentin in serum|
|Keywords:||antiepileptic drug, vigabatrin, gabapentin, micromethod, high-performance liquid chromatography (HPLC), GAMMA-AMINOBUTYRIC ACID, GAS-CHROMATOGRAPHY, PLASMA, URINE, PHARMACOKINETICS, ENANTIOMERS, ASSAY|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Neurology > Clinical and Experimental Epilepsy|
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