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A self-sustaining, light-entrainable circadian oscillator in the Drosophila brain.

Veleri, S; Brandes, C; Helfrich-Förster, C; Hall, JC; Stanewsky, R; (2003) A self-sustaining, light-entrainable circadian oscillator in the Drosophila brain. Curr Biol , 13 (20) pp. 1758-1767.

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Abstract

BACKGROUND: The circadian clock of Drosophila is able to drive behavioral rhythms for many weeks in continuous darkness (DD). The endogenous rhythm generator is thought to be generated by interlocked molecular feedback loops involving circadian transcriptional and posttranscriptional regulation of several clock genes, including period. However, all attempts to demonstrate sustained rhythms of clock gene expression in DD have failed, making it difficult to link the molecular clock models with the circadian behavioral rhythms. Here we restricted expression of a novel period-luciferase transgene to certain clock neurons in the Drosophila brain, permitting us to monitor reporter gene activity in these cells in real-time. RESULTS: We show that only a subset of the previously described pacemaker neurons is able to sustain PERIOD protein oscillations after 5 days in constant darkness. In addition, we identified a sustained and autonomous molecular oscillator in a group of clock neurons in the dorsal brain with heretofore unknown function. We found that these "dorsal neurons" (DNs) can synchronize behavioral rhythms and that light input into these cells involves the blue-light photoreceptor cryptochrome. CONCLUSIONS: Our results suggest that the DNs play a prominent role in controlling locomotor behavior when flies are exposed to natural light-dark cycles. Analysis of similar "stable mosaic" transgenes should help to reveal the function of the other clock neuronal clusters within the fly brain.

Type: Article
Title: A self-sustaining, light-entrainable circadian oscillator in the Drosophila brain.
Location: England
Keywords: Animals, Biological Clocks, Brain, Chromosome Mapping, Circadian Rhythm, Cryptochromes, Darkness, Drosophila, Drosophila Proteins, Eye Proteins, Flavoproteins, Gene Expression Profiling, Immunohistochemistry, Luciferases, Luminescent Measurements, Motor Activity, Neurons, Nuclear Proteins, Period Circadian Proteins, Photoperiod, Photoreceptor Cells, Invertebrate, Receptors, G-Protein-Coupled, Transgenes
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1431778
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