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A Two-Tier Golgi-Based Control of Organelle Size Underpins the Functional Plasticity of Endothelial Cells

Ferraro, F; Kriston-Vizi, J; Metcalf, DJ; Martin-Martin, B; Freeman, J; Burden, JJ; Westmoreland, D; ... Cutler, DF; + view all (2014) A Two-Tier Golgi-Based Control of Organelle Size Underpins the Functional Plasticity of Endothelial Cells. Developmental Cell , 29 (3) pp. 292-304. 10.1016/j.devcel.2014.03.021. Green open access

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Abstract

Weibel-Palade bodies (WPBs), endothelial-specific secretory granules that are central to primary hemostasis and inflammation, occur in dimensions ranging between 0.5 and 5 μm. How their size is determined and whether it has a functional relevance are at present unknown. Here, we provide evidence for a dual role of the Golgi apparatus in controlling the size of these secretory carriers. At the ministack level, cisternae constrain the size of nanostructures (“quanta”) of von Willebrand factor (vWF), the main WPB cargo. The ribbon architecture of the Golgi then allows copackaging of a variable number of vWF quanta within the continuous lumen of the trans-Golgi network, thereby generating organelles of different sizes. Reducing the WPB size abates endothelial cell hemostatic function by drastically diminishing platelet recruitment, but, strikingly, the inflammatory response (the endothelial capacity to engage leukocytes) is unaltered. Size can thus confer functional plasticity to an organelle by differentially affecting its activities.

Type: Article
Title: A Two-Tier Golgi-Based Control of Organelle Size Underpins the Functional Plasticity of Endothelial Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.devcel.2014.03.021
Publisher version: http://dx.doi.org/10.1016/j.devcel.2014.03.021
Language: English
Additional information: Copyright © 2014 The Authors. Published by Elsevier Inc.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
Keywords: Cell Biology, Developmental Biology, Von-willebrand-factor, Weibel-palade Bodies, Factor-deficient Mice, P-selectin, Vonwillebrand-factor, Cytoplasmic Components, Leukocyte Recruitment, Electron Tomography, Secretion, Trafficking
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1428926
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