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Monoclonal antibodies that distinguish between human aorta smooth muscle and endothelial cells.

Glukhova, MA; Kabakov, AY; Ornatsky, OI; Frid, MG; Smirnov, VN; (1985) Monoclonal antibodies that distinguish between human aorta smooth muscle and endothelial cells. FEBS Lett , 189 (2) pp. 291-295. 10.1016/0014-5793(85)81042-5.

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Abstract

A monoclonal antibody has been generated that interacts with the surface of cultured human aorta smooth muscle cells and does not bind to the endothelial cells from aorta and umbilical vein. An antigen recognized by the antibody has a molecular mass of 330 kDa as determined by electrophoresis of immunoprecipitate in SDS-polyacrylamide gel. The same antigen appeared to be present on the fibroblast surface while neither immunofluorescence, flow cytofluorimetry nor immunoprecipitation reveal it on the endothelial cell surface or in the Triton X-100 extract.

Type: Article
Title: Monoclonal antibodies that distinguish between human aorta smooth muscle and endothelial cells.
Location: England
DOI: 10.1016/0014-5793(85)81042-5
Keywords: Antibodies, Monoclonal, Antigens, Surface, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Endothelium, Flow Cytometry, Fluorescent Antibody Technique, Humans, Molecular Weight, Muscle, Smooth, Vascular, Octoxynol, Polyethylene Glycols
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1425288
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