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Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope.

Fernández-Alfonso, T; Nadella, KM; Iacaruso, MF; Pichler, B; Roš, H; Kirkby, PA; Silver, RA; (2014) Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope. J Neurosci Methods , 222 pp. 69-81. 10.1016/j.jneumeth.2013.10.021. Green open access

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Abstract

Two-photon microscopy is widely used to study brain function, but conventional microscopes are too slow to capture the timing of neuronal signalling and imaging is restricted to one plane. Recent development of acousto-optic-deflector-based random access functional imaging has improved the temporal resolution, but the utility of these technologies for mapping 3D synaptic activity patterns and their performance at the excitation wavelengths required to image genetically encoded indicators have not been investigated.

Type: Article
Title: Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.jneumeth.2013.10.021
Publisher version: http://dx.doi.org/10.1016/j.jneumeth.2013.10.021
Additional information: © 2013 The Authors. Published by Elsevier B.V. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: 3D functional imaging, Acousto-optic deflectors, Acousto-optic lens, Calcium imaging, Population imaging, Synaptic activity mapping, Two-photon imaging
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1422212
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