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Methods for recording and measuring tonic GABAA receptor-mediated inhibition.

Bright, DP; Smart, TG; (2013) Methods for recording and measuring tonic GABAA receptor-mediated inhibition. Front Neural Circuits , 7 , Article 193. 10.3389/fncir.2013.00193. Green open access

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Abstract

Tonic inhibitory conductances mediated by GABAA receptors have now been identified and characterized in many different brain regions. Most experimental studies of tonic GABAergic inhibition have been carried out using acute brain slice preparations but tonic currents have been recorded under a variety of different conditions. This diversity of recording conditions is likely to impact upon many of the factors responsible for controlling tonic inhibition and can make comparison between different studies difficult. In this review, we will firstly consider how various experimental conditions, including age of animal, recording temperature and solution composition, are likely to influence tonic GABAA conductances. We will then consider some technical considerations related to how the tonic conductance is measured and subsequently analyzed, including how the use of current noise may provide a complementary and reliable method for quantifying changes in tonic current.

Type: Article
Title: Methods for recording and measuring tonic GABAA receptor-mediated inhibition.
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fncir.2013.00193
Publisher version: http://dx.doi.org/10.3389/fncir.2013.00193
Additional information: © 2013 Bright and Smart. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. PMCID: PMC3852068
Keywords: GABAA, extrasynaptic, methods, noise, tonic inhibition
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1421931
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