ATP-DEPENDENT BRANCH MIGRATION OF HOLLIDAY JUNCTIONS PROMOTED BY THE RUVA AND RUVB PROTEINS OF ESCHERICHIA-COLI.
1171 - 1180.
The RuvA and RuvB proteins of E. coli, which are induced as part of the cellular response to DNA damage, act together to promote the branch migration of Holliday junctions. Addition of purified RuvA and RuvB to a RecA-mediated recombination reaction stimulates the rate of strand exchange and the formation of heteroduplex DNA. Stimulation does not occur via interaction with RecA; instead, RuvA and RuvB act directly upon recombination intermediates (Holliday junctions) made by RecA. We show that RuvAB-mediated branch migration requires ATP and can bypass UV-induced DNA lesions. At high RuvB concentrations, the requirement for RuvA is overcome, indicating that the RuvB ATPase provides the motor force for branch migration. RuvA protein provides specificity by binding to the Holliday junction, thereby reducing the requirement for RuvB by 50-fold. The newly discovered biochemical properties of RuvA, RuvB, and RuvC are incorporated into a model for the postreplicational repair of DNA following UV irradiation.
|Title:||ATP-DEPENDENT BRANCH MIGRATION OF HOLLIDAY JUNCTIONS PROMOTED BY THE RUVA AND RUVB PROTEINS OF ESCHERICHIA-COLI|
|Keywords:||STRAND EXCHANGE-REACTIONS, ESCHERICHIA-COLI, RECA PROTEIN, DNA-REPAIR, ENZYMATIC FORMATION, K-12 REVEALS, RECOMBINATION, PURIFICATION, RESOLUTION, MOLECULES|
|UCL classification:||UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Structural and Molecular Biology
Archive Staff Only