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Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex.

Beuth, B; García-Mayoral, MF; Taylor, IA; Ramos, A; (2007) Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex. J Am Chem Soc , 129 (33) pp. 10205-10210. 10.1021/ja072365q.

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Abstract

We describe a method to analyze the sequence specificity of an RNA-binding domain. The method, which we have named scaffold-independent analysis, reports on the specificity for each nucleotide position within an RNA target, uncoupled from the surrounding structural and sequence context. We expect this information to improve our understanding of protein-RNA interfaces in ssRNA binding domains (e.g., KH or RRM domains) and to be useful to the design of novel protein-RNA recognition surfaces. Our NMR binding assays using the third KH domain of the Nova-1 protein provide a proof-of-principle for the method and novel information on the specificity of this domain for its RNA targets.

Type: Article
Title: Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex.
Location: United States
DOI: 10.1021/ja072365q
Keywords: Antigens, Neoplasm, Humans, Nerve Tissue Proteins, Nuclear Magnetic Resonance, Biomolecular, Protein Structure, Tertiary, RNA, RNA-Binding Proteins
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1418612
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