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Inhibition of store-operated calcium entry contributes to the anti-proliferative effect of non-steroidal anti-inflammatory drugs in human colon cancer cells

Weiss, H; Amberger, A; Widschwendter, M; Margreiter, R; Ofner, D; Dietl, P; (2001) Inhibition of store-operated calcium entry contributes to the anti-proliferative effect of non-steroidal anti-inflammatory drugs in human colon cancer cells. INT J CANCER , 92 (6) 877 - 882.

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Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) inhibit proliferation and angiogenesis in colorectal cancer. We examined a possible involvement of store-operated calcium (SOC) entry in human colon carcinoma cells (HRT-18), which require calcium for proliferation. Acetyl-salicylic-ac id (ASA), mefenamic acid (MEF) and sulindac sulfide (SUS) inhibited cell proliferation with the following order of potency: SUS > MEF > > ASA, SUS but not MEF and ASA induced apoptosis following low-dose treatment, Furthermore, SUS and MEF significantly altered the cell cycle distribution. The ability of NSAIDs to inhibit SOC entry was assessed by measuring the intracellular calcium concentration ([Ca2+](i)) in response to calcium store depletion using the endoplasmic calcium ATPase inhibitor thapsigargin. SUS and MEF, but not ASA significantly inhibited SOC entry. A causal link between SOC entry inhibition and anti-proliferative activity was tested using the inorganic SOC entry inhibitor La3+ and the specific organic inhibitor N-1-n-octyl-3,5-bis-(4-pyridyl)triazole (DPT), Both La3+ and DPT inhibited cell proliferation and SOC entry. Analogous to MEF, the anti-proliferative effect of DPT was mediated by cell cycle arrest and not by induction of apoptosis, These data indicate a role of SOC entry for cell proliferation in cancer cells and suggest a novel anti-proliferative NSAID mechanism in addition to its known influence on lipid metabolism. (C) 2001 Wiley-Liss, Inc.

Type: Article
Title: Inhibition of store-operated calcium entry contributes to the anti-proliferative effect of non-steroidal anti-inflammatory drugs in human colon cancer cells
Keywords: anti-inflammatory drugs, calcium entry, calcium channel, colon cancer, cell cycle, apoptosis, proliferation, NONSTEROIDAL ANTIINFLAMMATORY DRUGS, CAPACITATIVE CA2+ ENTRY, FAMILIAL ADENOMATOUS POLYPOSIS, MEDIATED SIGNAL-TRANSDUCTION, COLORECTAL-CANCER, INTRACELLULAR CALCIUM, CARBOXYAMIDO-TRIAZOLE, ADENOCARCINOMA CELLS, CYTOPLASMIC CALCIUM, INFLUX INHIBITOR
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health > Women's Cancer
URI: http://discovery.ucl.ac.uk/id/eprint/141736
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