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Complexes of cytochrome P450 with metyrapone. A convenient method for the quantitative analysis of phenobarbital-inducible cytochrome P450 in rat liver microsomes.

Mitani, F; Shephard, EA; Phillips, IR; Rabin, BR; (1982) Complexes of cytochrome P450 with metyrapone. A convenient method for the quantitative analysis of phenobarbital-inducible cytochrome P450 in rat liver microsomes. FEBS Lett , 148 (2) pp. 302-306.

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Abstract

Phenobarbital-inducible cytochrome P450 variants have a higher affinity (app. Ks = 0.6 microM). The difference in affinity has enabled us to quantitate, by metyrapone-induced difference spectral analysis, phenobarbital-inducible cytochromes P450 in liver microsomal membranes. The result obtained by this simple, rapid technique compare well with those found by more sophisticated techniques such as radioimmunoassay and immunoprecipitation.

Type: Article
Title: Complexes of cytochrome P450 with metyrapone. A convenient method for the quantitative analysis of phenobarbital-inducible cytochrome P450 in rat liver microsomes.
Location: England
Keywords: Animals, Cytochrome P-450 Enzyme System, Kinetics, Male, Metyrapone, Microsomes, Liver, Phenobarbital, Rats, Rats, Inbred Strains, Spectrophotometry
URI: http://discovery.ucl.ac.uk/id/eprint/1413415
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