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Liquid chromatography using cellulosic continuous stationary phases

Velayudhan, AK; (1993) Liquid chromatography using cellulosic continuous stationary phases. In: Tsao, GT and Boyer, PM, (eds.) Chromatography. (147 - 160). Springer Berlin Heidelberg

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Abstract

A novel type of continuous stationary phase based on fabric materials is described. This column packing utilizes the continuous character of a cellulose (cotton) stationary phase, and the chemistry of the derivatized forms of the adsorbent, to obtain separations of proteins and small molecules based on cation and anion exchange, hydrophobic interactions, and size. The mechanical stability of the stationary phase facilitates chromatographic velocities in excess of 70 cm min−1. The influence of eluent properties on the adsorption of sample proteins is discussed in this chapter. Sequential stepwise desorption is used to separate 100 μl mixtures of BSA, IgG, β-galactosidase, and insulin in 10 minutes or less, using 10 mm i.d. × 500 mm length columns.

Type: Book chapter
Title: Liquid chromatography using cellulosic continuous stationary phases
ISBN-13: 978-3-540-56440-9
DOI: 10.1007/BFb0046576
Publisher version: http://link.springer.com/chapter/10.1007/BFb004657...
Language: English
Additional information: © 1993 Springer-Verlag
Keywords: Science
UCL classification: UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
URI: http://discovery.ucl.ac.uk/id/eprint/1413021
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