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A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes.

Shephard, EA; Pike, SF; Rabin, BR; Phillips, IR; (1983) A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes. Anal Biochem , 129 (2) pp. 430-433.

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Abstract

NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this single step procedure is as pure as that isolated by multicolumn procedures.

Type: Article
Title: A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes.
Location: United States
Keywords: Animals, Chromatography, Affinity, Cytochrome P-450 Enzyme System, Electrophoresis, Polyacrylamide Gel, Male, Microsomes, Liver, NADPH-Ferrihemoprotein Reductase, Radioimmunoassay, Rats, Rats, Inbred Strains
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1412997
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