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Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system.

Shiels, A; Phillips, I; Jeffery, S; Shephard, E; Carter, N; (1982) Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system. FEBS Lett , 148 (1) pp. 122-125.

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Abstract

Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS--polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same Mr-value as the native isoenzyme. CA III mRNA was found to represent approximately 0.55% of the total mRNA present in rat soleus muscle.

Type: Article
Title: Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system.
Location: England
Keywords: Animals, Carbonic Anhydrases, Cell-Free System, Electrophoresis, Polyacrylamide Gel, Immunologic Techniques, Muscles, Protein Biosynthesis, RNA, Radioimmunoassay, Rats
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1412719
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