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The effect of terpenoid compounds on cytochrome P-450 levels in rat liver.

Austin, CA; Shephard, EA; Pike, SF; Rabin, BR; Phillips, IR; (1988) The effect of terpenoid compounds on cytochrome P-450 levels in rat liver. Biochem Pharmacol , 37 (11) pp. 2223-2229.

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Abstract

We have investigated the ability of camphor, menthol, pinene, limonene and myrcene to induce in rats members of a cytochrome P-450 sub-family termed PB P-450. These proteins have recently been designated as members of the P450IIB sub-family. None of these naturally occurring terpenoids significantly changed the total content of cytochromes P-450 or cytochrome b5. Radioimmunoassay results showed that PB P-450 was induced 6-fold by camphor and to a lesser extent by menthol and pinene. The induction was confirmed by Western blotting. It was shown by nucleic acid hybridization that induction of PB P-450 by terpenoids was mediated by an increase in the amount of the corresponding mRNA. Analysis of the denaturation of mRNA-cDNA hybrids demonstrated that the mRNA induced by the terpenoids was encoded by a member of the P450IIB sub-family. None of the terpenoids had an effect on the amount of mRNA coding for P450IA2 (a cytochrome P-450 inducible by beta-naphthoflavone and isosafrole). The results indicate that cytochromes P-450 induced by a synthetic compound, phenobarbital, may have originally evolved in response to terpenoid compounds normally present in the environment.

Type: Article
Title: The effect of terpenoid compounds on cytochrome P-450 levels in rat liver.
Location: England
Keywords: Animals, Camphor, Cytochrome P-450 Enzyme System, Enzyme Induction, Menthol, Microsomes, Liver, Nucleic Acid Hybridization, Phenobarbital, RNA, Messenger, Rats, Rats, Inbred Strains, Terpenes
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1412520
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