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Measurement of ATP in Single Oocytes: Impact of Maturation and Cumulus Cells on Levels and Consumption

Dalton, CM; Szabadkai, G; Carroll, J; (2013) Measurement of ATP in Single Oocytes: Impact of Maturation and Cumulus Cells on Levels and Consumption. Journal of Cellular Physiology , 222 (3) pp. 353-361. 10.1002/jcp.24457. Green open access

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Abstract

Mitochondria provide the primary source of ATP in the oocyte and early embryo and mitochondrial dysfunction and deficit of mitochondria-derived ATP has been linked to suboptimal developmental competence. We have undertaken a study of ATP in the maturing mouse oocyte using a novel recombinant FRET based probe, AT1.03. We show that AT1.03 can be successfully used to monitor cytosolic ATP levels in single live oocytes over extended time periods. We find that ATP levels undergo dynamic changes associated with specific maturational events and that oocytes display altered rates of ATP consumption at different stages of maturation. Cumulus enclosed oocytes have a higher ATP level during maturation than denuded oocytes and this can be abolished by inhibition of gap junctional communication between the oocyte and cumulus cells. Our work uses a new approach to shed light on regulation of ATP levels and ATP consumption during oocyte maturation.

Type: Article
Title: Measurement of ATP in Single Oocytes: Impact of Maturation and Cumulus Cells on Levels and Consumption
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/jcp.24457
Publisher version: http://dx.doi.org/10.1002/jcp.24457
Language: English
Additional information: © 2013 Wiley Periodicals, Inc. Immunological Reviews published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License [http://creativecommons.org/licenses/by/3.0/], which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: ATP, mitochondria, oocyte
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1405344
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