Harvey, R;
Schuster, E;
Jennings, BH;
(2013)
Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila.
PLoS One
, 8
(7)
, Article e70184. 10.1371/journal.pone.0070184.
![[thumbnail of journal.pone.0070184.pdf]](https://discovery.ucl.ac.uk/style/images/fileicons/application_pdf.png) Preview |
PDF
journal.pone.0070184.pdf Download (4MB) |
Abstract
The early elongation checkpoint regulated by Positive Transcription Elongation Factor b (P-TEFb) is a critical control point for the expression of many genes. Spt5 interacts directly with RNA polymerase II and has an essential role in establishing this checkpoint, and also for further transcript elongation. Here we demonstrate that Drosophila Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho), and the majority of Pho binding sites overlap with Spt5 binding sites across the genome in S2 cells. Our results indicate that Pho can interact with Spt5 to regulate transcription elongation in a gene specific manner.
| Type: | Article |
|---|---|
| Title: | Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0070184 |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0070184 |
| Language: | English |
| Additional information: | © 2013 Harvey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3718797 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1400829 |
Archive Staff Only
 |
View Item |
