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Neuronal Recordings with Solid-Conductor Intracellular Nanoelectrodes (SCINEs)

Angle, MR; Schaefer, AT; (2012) Neuronal Recordings with Solid-Conductor Intracellular Nanoelectrodes (SCINEs). PLoS ONE , 7 (8) , Article e43194. 10.1371/journal.pone.0043194. Green open access

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Abstract

Direct electrical recording of the neuronal transmembrane potential has been crucial to our understanding of the biophysical mechanisms subserving neuronal computation. Existing intracellular recording techniques, however, limit the accuracy and duration of such measurements by changing intracellular biochemistry and/or by damaging the plasma membrane. Here we demonstrate that nanoengineered electrodes can be used to record neuronal transmembrane potentials in brain tissue without causing these physiological perturbations. Using focused ion beam milling, we have fabricated Solid-Conductor Intracellular NanoElectrodes (SCINEs), from conventional tungsten microelectrodes. SCINEs have tips that are <300 nm in diameter for several micrometers, but can be easily handled and can be inserted into brain tissue. Performing simultaneous whole-cell patch recordings, we show that SCINEs can record action potentials (APs) as well as slower, subthreshold neuronal potentials without altering cellular properties. These results show a key role for nanotechnology in the development of new electrical recording techniques in neuroscience.

Type: Article
Title: Neuronal Recordings with Solid-Conductor Intracellular Nanoelectrodes (SCINEs)
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0043194
Publisher version: http://dx.doi.org/10.1371/journal.pone.0043194
Language: English
Additional information: © Angle, Schaefer. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1399983
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