Carpenter, V and Matthews, K and Devlin, G and Stuart, S and Jensen, J and Conaglen, J and Jeanplong, F and Goldspink, P and Yang, SY and Goldspink, G and Bass, J and McMahon, C (2008) Mechano-growth factor reduces loss of cardiac function in acute myocardial infarction. HEART LUNG CIRC , 17 (1) 33 - 39. 10.1016/j.hlc.2007.04.013.
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Background: Mechano-growth factor (MGF) is a splice-variant of IGF-I sharing an identical mature region, but with a different E domain. Our objective was to determine if MGF would reduce the area of 'at-risk'myocardium and improve cardiac function in the post-infarct heart.Methods: Infarcts were induced by injection of microspheres. In experiment 1, sheep were treated with vehicle, 200 nM each of mature IGF-I, MGF E domain, or full-length MGE In experiment 2, sheep were treated with vehicle or 200 nM of MGF E domain alone. Cardiac function was assessed using echocardiography and sheep were killed eight days post-MI. Evans Blue dye was injected before death to stain the compromised myocardium. Immunohistochemistry was used to assess the abundance of pAkt(T308) and cleaved caspase 3.Results: In experiment 1, cardiac function improved in sheep treated with the MGF E domain, while in experiment 2, MGF E domain preserved cardiac function and there was 35% less compromised cardiac muscle than controls. Furthermore, immunostaining of cleaved caspase 3 was absent in MGF E domain-treated hearts, suggesting that MGF E domain reduced infarct expansion.Conclusions: We conclude that the E domain of MGF protects the myocardium against ischaemia, thus improving cardiac function post-MI.
|Title:||Mechano-growth factor reduces loss of cardiac function in acute myocardial infarction|
|Keywords:||myocardial infarction, myocardium, mechano-growth factor, MYOCYTE CELL-DEATH, FACTOR-I, IGF-I, EVANS BLUE, APOPTOSIS, MUSCLE, HEART, RATS, ACTIVATION, EXPRESSION|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)|
UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Child Health > Department of Genes, Development and Disease > ICH - Clinical and Molecular Genetics
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