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Mechano-growth factor reduces loss of cardiac function in acute myocardial infarction

Carpenter, V; Matthews, K; Devlin, G; Stuart, S; Jensen, J; Conaglen, J; ... McMahon, C; + view all (2008) Mechano-growth factor reduces loss of cardiac function in acute myocardial infarction. HEART LUNG CIRC , 17 (1) 33 - 39. 10.1016/j.hlc.2007.04.013.

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Abstract

Background: Mechano-growth factor (MGF) is a splice-variant of IGF-I sharing an identical mature region, but with a different E domain. Our objective was to determine if MGF would reduce the area of 'at-risk'myocardium and improve cardiac function in the post-infarct heart.Methods: Infarcts were induced by injection of microspheres. In experiment 1, sheep were treated with vehicle, 200 nM each of mature IGF-I, MGF E domain, or full-length MGE In experiment 2, sheep were treated with vehicle or 200 nM of MGF E domain alone. Cardiac function was assessed using echocardiography and sheep were killed eight days post-MI. Evans Blue dye was injected before death to stain the compromised myocardium. Immunohistochemistry was used to assess the abundance of pAkt(T308) and cleaved caspase 3.Results: In experiment 1, cardiac function improved in sheep treated with the MGF E domain, while in experiment 2, MGF E domain preserved cardiac function and there was 35% less compromised cardiac muscle than controls. Furthermore, immunostaining of cleaved caspase 3 was absent in MGF E domain-treated hearts, suggesting that MGF E domain reduced infarct expansion.Conclusions: We conclude that the E domain of MGF protects the myocardium against ischaemia, thus improving cardiac function post-MI.

Type:Article
Title:Mechano-growth factor reduces loss of cardiac function in acute myocardial infarction
DOI:10.1016/j.hlc.2007.04.013
Keywords:myocardial infarction, myocardium, mechano-growth factor, MYOCYTE CELL-DEATH, FACTOR-I, IGF-I, EVANS BLUE, APOPTOSIS, MUSCLE, HEART, RATS, ACTIVATION, EXPRESSION
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Neurology > Clinical Neuroscience
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)

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