UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers

Rakowska, PD; Jiang, H; Ray, S; Pyne, A; Lamarre, B; Carr, M; Judge, PJ; ... Ryadnov, MG; + view all (2013) Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers. Proceedings of the National Academy of Sciences , 110 (22) 8918 - 8923. 10.1073/pnas.1222824110. Green open access

[img]
Preview
Text (Article)
Hoogenboom_Rakowksa2013.pdf

Download (1MB) | Preview
[img]
Preview
Text (Supporting Information)
Hoogenboom_Revised_PNAS_SI.pdf

Download (4MB) | Preview

Abstract

Antimicrobial peptides are postulated to disrupt microbial phospholipid membranes. The prevailing molecular model is based on the formation of stable or transient pores although the direct observation of the fundamental processes is lacking. By combining rational peptide design with topographical (atomic force microscopy) and chemical (nanoscale secondary ion mass spectrometry) imaging on the same samples, we show that pores formed by antimicrobial peptides in supported lipid bilayers are not necessarily limited to a particular diameter, nor they are transient, but can expand laterally at the nano-to-micrometer scale to the point of complete membrane disintegration. The results offer a mechanistic basis for membrane poration as a generic physicochemical process of cooperative and continuous peptide recruitment in the available phospholipid matrix.

Type: Article
Title: Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.1222824110
Publisher version: http://dx.doi.org/10.1073/pnas.1222824110
Language: English
Additional information: Copyright © The Authors 2013.
Keywords: antibiotics, de novo protein design, innate host defense, nanometrology, nanoscopy, Amino Acid Sequence, Antimicrobial Cationic Peptides, Chromatography, High Pressure Liquid, Circular Dichroism, Lipid Bilayers, Magnetic Resonance Spectroscopy, Mass Spectrometry, Microscopy, Atomic Force, Molecular Dynamics Simulation, Molecular Sequence Data, Nanotechnology, Phospholipids, Protein Engineering, Spectrometry, Mass, Secondary Ion
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1393881
Downloads since deposit
128Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item