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The G protein alpha subunit has a key role in determining the specificity of coupling to, but not the activation of, G protein-gated inwardly rectifying K+ channels

Leaney, JL; Milligan, G; Tinker, A; (2000) The G protein alpha subunit has a key role in determining the specificity of coupling to, but not the activation of, G protein-gated inwardly rectifying K+ channels. J BIOL CHEM , 275 (2) 921 - 929. Gold open access

Abstract

In neuronal and atrial tissue, G protein-gated inwardly rectifying K+ channels (Kir3.x family) are responsible for mediating inhibitory postsynaptic potentials and slowing the heart rate, They are activated by G beta gamma dimers released in response to the stimulation of receptors coupled to inhibitory G proteins of the G(i/o) family but not receptors coupled to the stimulatory G protein G(s), We have used biochemical, electrophysiological, and molecular biology techniques to examine this specificity of channel activation. In this study we have succeeded in reconstituting such specificity in an heterologous expression system stably expressing a cloned counterpart of the neuronal channel (Kir3.1 and Kir3.2A heteromultimers), The use of pertussis toxin-resistant G protein alpha subunits and chimeras between G(il) and G(s) indicate a central role for the G protein alpha subunits in determining receptor specificity of coupling to, but not activation of, G protein-gated inwardly rectifying K+ channels.

Type:Article
Title:The G protein alpha subunit has a key role in determining the specificity of coupling to, but not the activation of, G protein-gated inwardly rectifying K+ channels
Open access status:An open access publication
Publisher version:http://www.jbc.org/content/early/recent/0
Keywords:BETA-GAMMA-SUBUNITS, CELL-FREE MEMBRANE, I-KACH, POTASSIUM CHANNEL, BINDING-PROTEINS, XENOPUS OOCYTES, MOLECULAR-PROPERTIES, MUSCARINIC RECEPTOR, STRUCTURAL ELEMENTS, ADENYLYL-CYCLASE
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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