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Isolation of mouse hepatocytes.

Edwards, M; Houseman, L; Phillips, IR; Shephard, EA; (2013) Isolation of mouse hepatocytes. Methods Mol Biol , 987 283 - 293. 10.1007/978-1-62703-321-3_24.

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Abstract

Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital.

Type: Article
Title: Isolation of mouse hepatocytes.
Location: United States
DOI: 10.1007/978-1-62703-321-3_24
Language: English
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1389933
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