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Quantification of cell cycle-arresting proteins.

Kepp, O; Martins, I; Menger, L; Michaud, M; Adjemian, S; Sukkurwala, AQ; Galluzzi, L; (2013) Quantification of cell cycle-arresting proteins. Methods Mol Biol , 965 pp. 121-142. 10.1007/978-1-62703-239-1_7.

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Abstract

Cellular senescence, which can be defined as a stress response preventing the propagation of cells that have accumulated potentially oncogenic alterations, is invariably associated with a permanent cell cycle arrest. Such an irreversible blockage is mainly mediated by the persistent upregulation of one or more cyclin-dependent kinase inhibitors (CKIs), including (though not limited to) p16( INK4A ) and p21( CIP1 ) and p27( KIP1 ). CKIs operate by binding to cyclin-dependent kinases (CDKs), de facto inhibiting their enzymatic activity. Here, we provide an immunoblotting-based method for the detection and quantification of CKIs in vitro and ex vivo, together with a set of guidelines for the interpretation of results.

Type: Article
Title: Quantification of cell cycle-arresting proteins.
Location: United States
DOI: 10.1007/978-1-62703-239-1_7
Keywords: Cell Cycle Checkpoints, Cellular Senescence, Cyclin-Dependent Kinase Inhibitor Proteins, Electrophoresis, Gene Expression Regulation, HeLa Cells, Humans, Immunoblotting
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/1389849
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