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Rab7A Is Required for Efficient Production of Infectious HIV-1

Caillet, M; Janvier, K; Pelchen-Matthews, A; Delcroix-Genete, D; Camus, G; Marsh, M; Berlioz-Torrent, C; (2011) Rab7A Is Required for Efficient Production of Infectious HIV-1. PLoS Pathogens , 7 (11) , Article e1002347. 10.1371/journal.ppat.1002347. Green open access

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Abstract

Retroviruses take advantage of cellular trafficking machineries to assemble and release new infectious particles. Rab proteins regulate specific steps in intracellular membrane trafficking by recruiting tethering, docking and fusion factors, as well as the actin- and microtubule-based motor proteins that facilitate vesicle traffic. Using virological tests and RNA interference targeting Rab proteins, we demonstrate that the late endosome-associated Rab7A is required for HIV-1 propagation. Analysis of the late steps of the HIV infection cycle shows that Rab7A regulates Env processing, the incorporation of mature Env glycoproteins into viral particles and HIV-1 infectivity. We also show that siRNA-mediated Rab7A depletion induces a BST2/Tetherin phenotype on HIV-1 release. BST2/Tetherin is a restriction factor that impedes HIV-1 release by tethering mature virus particles to the plasma membrane. Our results suggest that Rab7A contributes to the mechanism by which Vpu counteracts the restriction factor BST2/Tetherin and rescues HIV-1 release. Altogether, our results highlight new roles for a major regulator of the late endocytic pathway, Rab7A, in the late stages of the HIV-1 replication cycle.

Type: Article
Title: Rab7A Is Required for Efficient Production of Infectious HIV-1
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.ppat.1002347
Publisher version: http://dx.doi.org/10.1371/journal.ppat.1002347
Language: English
Additional information: © 2011 Caillet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health
URI: http://discovery.ucl.ac.uk/id/eprint/1389269
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