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Chlamydiae Assemble a Pathogen Synapse to Hijack the Host Endoplasmic Reticulum

Dumoux, M; Clare, DK; Saibil, HR; Hayward, RD; (2012) Chlamydiae Assemble a Pathogen Synapse to Hijack the Host Endoplasmic Reticulum. Traffic , 13 (12) pp. 1612-1627. 10.1111/tra.12002. Green open access

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Abstract

Chlamydiae are obligate intracellular bacterial pathogens that replicate within a specialized membrane-bound compartment, termed an ‘inclusion’. The inclusion membrane is a critical host–pathogen interface, yet the extent of its interaction with cellular organelles and the origin of this membrane remain poorly defined. Here we show that the host endoplasmic reticulum (ER) is specifically recruited to the inclusion, and that key rough ER (rER) proteins are enriched on and translocated into the inclusion. rER recruitment is a Chlamydia-orchestrated process that occurs independently of host trafficking. Generation of infectious progeny requires an intact ER, since ER vacuolation early during infection stalls inclusion development, whereas disruption post ER recruitment bursts the inclusion. Electron tomography and immunolabelling of Chlamydia-infected cells reveal ‘pathogen synapses’ at which ordered arrays of chlamydial type III secretion complexes connect to the inclusion membrane only at rER contact sites. Our data show a supramolecular assembly involved in pathogen hijack of a key host organelle.

Type: Article
Title: Chlamydiae Assemble a Pathogen Synapse to Hijack the Host Endoplasmic Reticulum
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/tra.12002
Publisher version: http://dx.doi.org/10.1111/tra.12002
Language: English
Additional information: Re-use of this article is permitted in accordance with theTerms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms
Keywords: CELL BIOLOGY, Chlamydia, endoplasmic reticulum, microscopy, pathogenesis, type III secretion, SMALL-MOLECULE INHIBITOR, SECRETION SYSTEM NEEDLE, III SECRETION, TRACHOMATIS, PROTEIN, MEMBRANE, COMPLEX, CELL, TRAFFICKING, TOMOGRAPHY
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1387836
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