UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Analytical strategies for characterization of oxysterol lipidomes: Liver X receptor ligands in plasma

Griffiths, WJ; Crick, PJ; Wang, Y; Ogundare, M; Tuschl, K; Morris, AA; Bigger, BW; ... Wang, Y; + view all (2013) Analytical strategies for characterization of oxysterol lipidomes: Liver X receptor ligands in plasma. Free Radical Biology and Medicine , 59 69 - 84. 10.1016/j.freeradbiomed.2012.07.027. Green open access

[img]
Preview
PDF
1376362.pdf
Available under License : See the attached licence file.

Download (948kB)

Abstract

Bile acids, bile alcohols, and hormonal steroids represent the ultimate biologically active products of cholesterol metabolism in vertebrates. However, intermediates in their formation, including oxysterols and cholestenoic acids, also possess known, e.g., as ligands to nuclear and G-protein-coupled receptors, and unknown regulatory activities. The potential diversity of molecules originating from the cholesterol structure is very broad and their abundance in biological materials ranges over several orders of magnitude. Here we describe the application of enzyme-assisted derivatization for sterol analysis (EADSA) in combination with liquid chromatography–electrospray ionization–mass spectrometry to define the oxysterol and cholestenoic acid metabolomes of human plasma. Quantitative profiling of adult plasma using EADSA leads to the detection of over 30 metabolites derived from cholesterol, some of which are ligands to the nuclear receptors LXR, FXR, and pregnane X receptor or the G-protein-coupled receptor Epstein–Barr virus-induced gene 2. The potential of the EADSA technique in screening for inborn errors of cholesterol metabolism and biosynthesis is demonstrated by the unique plasma profile of patients suffering from cerebrotendinous xanthomatosis. The analytical methods described are easily adapted to the analysis of other biological fluids, including cerebrospinal fluid, and also tissues, e.g., brain, in which nuclear and G-protein-coupled receptors may have important regulatory roles.

Type: Article
Title: Analytical strategies for characterization of oxysterol lipidomes: Liver X receptor ligands in plasma
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.freeradbiomed.2012.07.027
Publisher version: http://dx.doi.org/10.1016/j.freeradbiomed.2012.07....
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PubMed ID: 22846477
Keywords: Lipidomics, Sterol, Steroid, Oxysterol, Liquid chromatography, Mass spectrometry, Bile acid, Derivatization, Cerebrotendinous xanthomatosis, Nuclear receptor, G-protein-coupled receptor, Liver X receptor, Free radicals
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Genetics and Genomic Medicine Prog
URI: http://discovery.ucl.ac.uk/id/eprint/1376362
Downloads since deposit
215Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item