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Tryptophan metabolism in vitamin B6-deficient mice

Bender, DA; Njagi, EN; Danielian, PS; (1990) Tryptophan metabolism in vitamin B6-deficient mice. British Journal of Nutrition , 63 (1) 27 - 36. 10.1079/BJN19900089. Green open access

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Abstract

Vitamin B6 deficiency was induced in mice by maintenance for 4 weeks on a vitamin B6-free diet. Tryptophan metabolism was assessed by determining the urinary excretion of tryptophan metabolites, the metabolism of [14C]tryptophan in vivo and the formation of tryptophan and niacin metabolites by isolated hepatocytes. The vitamin B6-deficient animals excreted more xanthurenic acid and 3-hydroxykynurenine, and less of the niacin metabolites N1-methyl nicotinamide and methyl-2-pyridone-4-carboxamide, than did control animals maintained on the same diet supplemented with 5 mg vitamin B6/kg. After intraperitoneal injection of [14C]tryptophan, vitamin B6-deficient mice showed lower liberation of 14CO2 from [methylene-14C]tryptophan and [U-14C]tryptophan than did controls, indicating impairment of kynureninase (EC 3.7.1.3) activity. There was no difference between the two groups of animals in the metabolism of [ring-2-14C]tryptophan. Hepatocytes isolated from the vitamin B6-deficient animals formed more 3-hydroxykynurenine and xanthurenic acid than did cells from control animals, but also formed more NADP and free niacin.

Type: Article
Title: Tryptophan metabolism in vitamin B6-deficient mice
Open access status: An open access version is available from UCL Discovery
DOI: 10.1079/BJN19900089
Publisher version: http://dx.doi.org/10.1079/BJN19900089
Language: English
Additional information: © 1990 Cambridge University Press
Keywords: Alanine, metabolism, Animals, Cells, Cultured, Formic Acids, Liver, Male, Mice, Inbred Strains, Tryptophan, Vitamin B 6 Deficiency, urine, Weight Gain, physiology
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/137510
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