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Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart.

Schirmer, JM; Miyagi, N; Rao, VP; Ricci, D; Federspiel, MJ; Kotin, RM; Russell, SJ; (2007) Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart. Transpl Int , 20 (6) pp. 550-557. 10.1111/j.1432-2277.2007.00479.x.

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Abstract

Efficient durable viral vector transduction of the transplanted heart remains elusive. This study assesses the potential of recombinant adeno-associated virus (rAAV) mediated gene delivery to the transplanted rat heart. rAAV serotype 1, 2 and 5 vectors encoding the green fluorescent protein (GFP) gene (1 x 10(11) viral particles/ml) were diluted in cold University of Wisconsin solution and circulated through the coronary vasculature of the donor organs for 30 min before syngeneic rat heterotopic heart transplantation was performed. Study 1: animals (n = 5 each serotype) were killed at 21 days post-transplant to evaluate the efficiency of GFP transduction using RT-PCR and expression by fluorescence microscopy. Study 2: using rAAV-1, animals (n = 5 each group) were killed at 7, 21 and 84 days to evaluate the durability of GFP expression. The maximum cardiac GFP expression at 21 days was observed in rAAV-1. GFP expression by rAAV-1 was detectable at 7 days, improved at 21 days, and was still evident at 84 days. This study demonstrates cardiac rAAV gene transduction with a cold perfusion preservation system of the donor heart. These data show that AAV-1 is superior to AAV-2 and AAV-5 for this purpose and that durable expression is achievable.

Type: Article
Title: Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart.
Location: England
DOI: 10.1111/j.1432-2277.2007.00479.x
Keywords: Adenoviridae, Animals, Gene Expression, Genetic Vectors, Green Fluorescent Proteins, Heart Transplantation, Male, Rats, Rats, Inbred Lew, Recombination, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Transduction, Genetic
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute of Cardiovascular Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute of Cardiovascular Science > Pre-clinical and Fundamental Science
URI: http://discovery.ucl.ac.uk/id/eprint/1373704
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