Molecular cloning and characterization of the platelet-activating factor receptor gene expressed in the human heart.
Biochem Biophys Res Commun
PAF decreases cardiac contractility and blood pressure. To characterize the cardiac PAF receptor, we screened a human ventricular cDNA library in a low stringency condition, using a PCR product derived from guinea pig lung PAF receptor as a probe. Four clones were obtained and named HV1-4. In Xenopus oocytes injected with cRNA derived from HV3 or 4 but not from HV1 or 2, PAF elicited a Ca(2+)-activated Cl- current. HV3 and HV4 were duplicate clones, encoding a 342 amino-acid polypeptide which was identical to that of the human leukocyte PAF receptor. However, a portion of the 5' untranslated region of HV3 (or 4) was different from that of the leukocyte receptor cDNA. Northern blotting of human ventricles and atria using the HV3 insert showed a single band of approximately 4 kb. These results suggest a tissue-specific translational mechanism responsible for regulation of the expression of the PAF receptor mRNA in these tissues.
|Title:||Molecular cloning and characterization of the platelet-activating factor receptor gene expressed in the human heart.|
|Keywords:||Amino Acid Sequence, Animals, Azepines, Base Sequence, Blotting, Northern, Cloning, Molecular, DNA, Electrophysiology, Gene Expression, Heart, Heart Ventricles, Humans, Male, Middle Aged, Molecular Sequence Data, Oligodeoxyribonucleotides, Oocytes, Platelet Activating Factor, Platelet Membrane Glycoproteins, Polymerase Chain Reaction, RNA, Receptors, Cell Surface, Receptors, G-Protein-Coupled, Triazoles, Xenopus|
|UCL classification:||UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science
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