Richardson, S; Siow, B; Batchelor, AM; Lythgoe, MF; Alexander, DC; (2013) A viable isolated tissue system: a tool for detailed MR measurements and controlled perturbation in physiologically stable tissue. Magn Reson Med , 69 (6) 1603 - 1610. 10.1002/mrm.24410.
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In vivo magnetic resonance imaging (MRI) assessment of neuronal tissue is prone to artifacts such as movement, pulsatile flow, and tissue susceptibility. Furthermore, stable in vivo scans of over 3 h are difficult to achieve, experimental design is therefore limited. Using isolated tissue maintained in a viable physiological state can mitigate many of these in vivo issues. This work describes the fabrication and validation of an MRI compatible viable isolated tissue maintenance chamber. Parameters measured from maintained rat optic nerves did not change significantly over 10 h: (i) mean axon radius [electron microscopy--0 h: 0.75±0.46; 5 h: 0.74±0.35; 10 h: 0.76±0.35 μm (P>0.05, t-test], (ii) action potentials [grease-gap electrophysiology--4.89±0.16 mv, (P>0.05, Pearson test], and (iii) diffusion tensor imaging parameters [fractional anisotropy: 0.86±0.02 (P>0.05, Pearson test), mean diffusivity: 1.48E-06±9.74E-08 cm2/s, (P>0.05, Pearson test)]. In addition, a thorough diffusion-weighted MR protocol demonstrated the comparable stability of viable isolated and chemically fixed rat optic nerve. This MRI compatible viable isolated tissue system allows researchers to probe neuronal physiology in a controlled environment by limiting in vivo artifacts and allowing extended MRI acquisitions.
|Title:||A viable isolated tissue system: a tool for detailed MR measurements and controlled perturbation in physiologically stable tissue.|
|Keywords:||Animals, Environment, Controlled, Equipment Design, Equipment Failure Analysis, Magnetic Resonance Imaging, Optic Nerve, Organ Culture Techniques, Perfusion, Rats, Rats, Sprague-Dawley, Reproducibility of Results, Rheology, Sensitivity and Specificity|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)|
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of) > Metabolism and Experimental Therapeutics
UCL > School of BEAMS > Faculty of Engineering Science > Computer Science
UCL > School of BEAMS > Faculty of Engineering Science > Medical Physics and Bioengineering
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