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Simple methods for generating neural, bone and endodermal cell types from chick embryonic stem cells

Boast, S; Stern, CD; (2013) Simple methods for generating neural, bone and endodermal cell types from chick embryonic stem cells. Stem Cell Research , 10 (1) 20 - 28. 10.1016/j.scr.2012.08.008.

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Abstract

Most work on embryonic stem cell differentiation uses mammalian cells derived from the blastocyst stage and some of the most widely used protocols to induce differentiation involve growing these cells in monolayer culture. Equivalent stem cells can be obtained from embryos of non-mammalian vertebrates, but to date this has only been successful in birds. These cells can contribute to all somatic lineages in chimaeras and can be induced to differentiate into a variety of cell types in vitro via embryoid body formation. However to date there are no reliable methods for differentiating them into descendants from each of the germ layers in monolayer culture, comparable to the protocols used in mammals. Here we describe three simple and reproducible protocols for differentiation of chick embryonic stem cells into mesoderm (bone), endoderm and neuroectoderm (neurons and glia) in monolayer culture. These methods open the way for more direct comparisons of the properties of mammalian and avian embryonic stem cells that may highlight similarities and differences.

Type: Article
Title: Simple methods for generating neural, bone and endodermal cell types from chick embryonic stem cells
Location: England
DOI: 10.1016/j.scr.2012.08.008
Publisher version: http://dx.doi.org/ 10.1016/j.scr.2012.08.008
Language: English
Keywords: Animals, Cell Culture Techniques, Cell Differentiation, Cells, Cultured, Chickens, Ectoderm, Embryonic Stem Cells, Endoderm, Mesoderm
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1366712
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