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Imaging Surface Charges of Individual Biomolecules

Leung, C; Kinns, H; Hoogenboom, BW; Howorka, S; Mesquida, P; (2009) Imaging Surface Charges of Individual Biomolecules. NANO LETT , 9 (7) 2769 - 2773. 10.1021/nl9012979.

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Abstract

Surface charges play a key role in determining the structure and function of proteins, DNA, and larger biomolecular structures.(1-4) Here we report on the measurement of the electrostatic surface potential of individual DNA and avidin molecules with nanometer resolution using Kelvin probe force microscopy. We also show, for the first time, the surface potential of buffer salts shielding individual DNA molecules, which would not be possible with conventional ensemble techniques.

Type:Article
Title:Imaging Surface Charges of Individual Biomolecules
DOI:10.1021/nl9012979
Keywords:PROBE FORCE MICROSCOPY, PULMONARY SURFACTANT, ULTRAHIGH-VACUUM, LABEL-FREE, AVIDIN, VISUALIZATION, MEMBRANE, LEVEL, FILMS
UCL classification:UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry
UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Physics and Astronomy

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