UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

The spatial order of transcription in mammalian cells.

Levsky, JM; Shenoy, SM; Chubb, JR; Hall, CB; Capodieci, P; Singer, RH; (2007) The spatial order of transcription in mammalian cells. J Cell Biochem , 102 (3) pp. 609-617. 10.1002/jcb.21495.

Full text not available from this repository.


We have previously developed technology for multiplexing probes for the detection of transcription of many genes simultaneously within single cells. This has allowed us to determine the spatial localization of multiple genes with respect to each other in the nucleus, and ultimately the expression profile of the cell with respect to surrounding cells in a tissue. Six parameters of transcriptional organization in individual cells from culture and tissue were used to characterize significant differences in intracellular and intercellular expression patterns while preserving cellular morphology and histological context. We found that, unlike yeast, mammalian expression is excluded from the periphery and in addition, a subtle but complex organization underlies the transcriptional activity of these cells, both intra- and intercellularly. The approach has sufficient spatial resolution to be applied to the detection of chromosomal translocations or the identification of cancer cells.

Type: Article
Title: The spatial order of transcription in mammalian cells.
Location: United States
DOI: 10.1002/jcb.21495
Keywords: Cell Line, Tumor, Chromatin, Cluster Analysis, Gene Expression Profiling, Gene Expression Regulation, Humans, In Situ Hybridization, Fluorescence, Microscopy, Models, Biological, Models, Genetic, Molecular Biology, Transcription, Genetic, Translocation, Genetic
URI: http://discovery.ucl.ac.uk/id/eprint/1364199
Downloads since deposit
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item