UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Image analysis for quantification of fungal biomass and differentiation

Thomas, CR; Paul, GC; Kent, CA; Packer, HL; Keshavarz-Moore, E; Lilly, MD; (1992) Image analysis for quantification of fungal biomass and differentiation. IFAC Symposia Series (10) pp. 41-44.

Full text not available from this repository.

Abstract

Fully-automatic image analysis methods are described for the measurement of vacuolation in filamentous fungi growing in dispersed form in submerged cultures. For Penicillium chrysogenum biomass from 0.03 to 38 gL-L, volumes of cytoplasmic and vacuolized or degenerated regions can be converted successfully into dry weight estimations, even when the medium contains suspended solids up to 30 gL-1. The vacuolation can be characterized in detail for physiological studies. For example, size and shape distributions of vacuoles can be measured, and are presented across the time course of a test fed-batch penicillin fermentation. Although off-line, the method is rapid enough (30 min per sample) to be considered for use as a process control tool. Links between cytoplasmic content and antibiotic production are being sought. The method might be used to provide data for structured models of fungal fermentations, and for control of product formation in those fermentations.

Type: Article
Title: Image analysis for quantification of fungal biomass and differentiation
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/1361969
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item