UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Biochemical characterization and regulation of cardiolipin synthase in Saccharomyces cerevisiae.

Tamai, KT; Greenberg, ML; (1990) Biochemical characterization and regulation of cardiolipin synthase in Saccharomyces cerevisiae. Biochim Biophys Acta , 1046 (2) pp. 214-222. 10.1016/0005-2760(90)90192-z.

Full text not available from this repository.

Abstract

Cardiolipin (CL) synthase activity was characterized in mitochondrial extracts of the yeast Saccharomyces cerevisiae and was shown for the first time to utilize CDP-diacylglycerol as a substrate. CL synthase exhibited a pH optimum of 9.0. Maximal activity was obtained in the presence of 20 mM magnesium with a Triton X-100: phospholipid ratio of 1:1. The apparent Km values for phosphatidylglycerol and CDP-diacylglycerol were 1 mM and 36 microM, respectively. CL synthase activity was maximal at 45 degrees C and heat inactivation studies showed that the enzyme retained greater than 75% of its activity at temperatures up to 55 degrees C. To study the regulation of CL synthase, the enzyme was assayed in cells grown under conditions known to affect general phospholipid synthesis. Unlike many phospholipid biosynthetic enzymes including PGP synthase, which catalyzes the initial step in CL biosynthesis, CL synthase was not repressed in cells grown in the presence of the phospholipid precursor inositol. Detailed procedures for the enzymatic synthesis of 32P-labelled substrates are described.

Type: Article
Title: Biochemical characterization and regulation of cardiolipin synthase in Saccharomyces cerevisiae.
Location: Netherlands
DOI: 10.1016/0005-2760(90)90192-z
Keywords: Cytidine Diphosphate Diglycerides, Enzyme Stability, Hydrogen-Ion Concentration, Inositol, Kinetics, Magnesium, Membrane Proteins, Mitochondria, Octoxynol, Phosphatidylglycerols, Phosphotransferases, Polyethylene Glycols, Saccharomyces cerevisiae, Substrate Specificity, Temperature, Transferases (Other Substituted Phosphate Groups)
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1359375
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item