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Equilibrium unfolding thermodynamics of beta2-microglobulin analyzed through native-state H/D exchange.

Rennella, E; Corazza, A; Fogolari, F; Viglino, P; Giorgetti, S; Stoppini, M; Bellotti, V; (2009) Equilibrium unfolding thermodynamics of beta2-microglobulin analyzed through native-state H/D exchange. Biophys J , 96 (1) pp. 169-179. 10.1529/biophysj.108.142448.

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Abstract

The exchange rates for the amide hydrogens of beta(2)-microglobulin, the protein responsible for dialysis-related amyloidosis, were measured under native conditions at different temperatures ranging from 301 to 315 K. The pattern of protection factors within different regions of the protein correlates well with the hydrogen-bonding pattern of the deposited structures. Analysis of the exchange rates indicates the presence of mixed EX1- and EX2-limit mechanisms. The measured parameters are consistent with a two-process model in which two competing pathways, i.e., global unfolding in the core region and partial openings of the native state, determine the observed exchange rates. These findings are analyzed with respect to the amyloidogenic properties of the protein.

Type: Article
Title: Equilibrium unfolding thermodynamics of beta2-microglobulin analyzed through native-state H/D exchange.
Location: United States
DOI: 10.1529/biophysj.108.142448
Keywords: Algorithms, Amino Acid Sequence, Deuterium Exchange Measurement, Hydrogen, Hydrogen Bonding, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Protein Conformation, Protein Folding, Recombinant Proteins, Temperature, Thermodynamics, Water, beta 2-Microglobulin
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/1356523
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