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Design of high productivity antibody capture by protein A chromatography using an integrated experimental and modeling approach.

Ng, CK; Osuna-Sanchez, H; Valéry, E; Sørensen, E; Bracewell, DG; (2012) Design of high productivity antibody capture by protein A chromatography using an integrated experimental and modeling approach. Journal of Chromatography B , 899 116 - 126. 10.1016/j.jchromb.2012.05.010. Green open access

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Abstract

An integrated experimental and modeling approach for the design of high productivity protein A chromatography is presented to maximize productivity in bioproduct manufacture. The approach consists of four steps: (1) small-scale experimentation, (2) model parameter estimation, (3) productivity optimization and (4) model validation with process verification. The integrated use of process experimentation and modeling enables fewer experiments to be performed, and thus minimizes the time and materials required in order to gain process understanding, which is of key importance during process development. The application of the approach is demonstrated for the capture of antibody by a novel silica-based high performance protein A adsorbent named AbSolute. In the example, a series of pulse injections and breakthrough experiments were performed to develop a lumped parameter model, which was then used to find the best design that optimizes the productivity of a batch protein A chromatographic process for human IgG capture. An optimum productivity of 2.9 kg L⁻¹ day⁻¹ for a column of 5mm diameter and 8.5 cm length was predicted, and subsequently verified experimentally, completing the whole process design approach in only 75 person-hours (or approximately 2 weeks).

Type: Article
Title: Design of high productivity antibody capture by protein A chromatography using an integrated experimental and modeling approach.
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.jchromb.2012.05.010
Publisher version: http://dx.doi.org/10.1016/j.jchromb.2012.05.010
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Adsorption, Chromatography, Affinity, Humans, Immunoglobulin G, Models, Theoretical, Staphylococcal Protein A
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/1356197
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