UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly.

Baranova, E; Fronzes, R; Garcia-Pino, A; Van Gerven, N; Papapostolou, D; Péhau-Arnaudet, G; ... Remaut, H; + view all (2012) SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly. Nature , 487 (7405) 119 - 122. 10.1038/nature11155.

Full text not available from this repository.

Abstract

S-layers are regular two-dimensional semipermeable protein layers that constitute a major cell-wall component in archaea and many bacteria. The nanoscale repeat structure of the S-layer lattices and their self-assembly from S-layer proteins (SLPs) have sparked interest in their use as patterning and display scaffolds for a range of nano-biotechnological applications. Despite their biological abundance and the technological interest in them, structural information about SLPs is limited to truncated and assembly-negative proteins. Here we report the X-ray structure of the SbsB SLP of Geobacillus stearothermophilus PV72/p2 by the use of nanobody-aided crystallization. SbsB consists of a seven-domain protein, formed by an amino-terminal cell-wall attachment domain and six consecutive immunoglobulin-like domains, that organize into a φ-shaped disk-like monomeric crystallization unit stabilized by interdomain Ca(2+) ion coordination. A Ca(2+)-dependent switch to the condensed SbsB quaternary structure pre-positions intermolecular contact zones and renders the protein competent for S-layer assembly. On the basis of crystal packing, chemical crosslinking data and cryo-electron microscopy projections, we present a model for the molecular organization of this SLP into a porous protein sheet inside the S-layer. The SbsB lattice represents a previously undescribed structural model for protein assemblies and may advance our understanding of SLP physiology and self-assembly, as well as the rational design of engineered higher-order structures for biotechnology.

Type:Article
Title:SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly.
Location:England
DOI:10.1038/nature11155
Language:English
Keywords:Bacterial Proteins, Calcium, Cryoelectron Microscopy, Crystallization, Crystallography, X-Ray, Geobacillus stearothermophilus, Immunoglobulins, Membrane Proteins, Models, Molecular, Molecular Dynamics Simulation, Nanostructures, Polymerization, Protein Structure, Quaternary, Protein Structure, Tertiary, Solutions
UCL classification:UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry

Archive Staff Only: edit this record