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Isolation and identification of a cDNA clone coding for an HLA-DR transplantation antigen alpha-chain.

Gustafsson, K; Bill, P; Larhammar, D; Wiman, K; Claesson, L; Schenning, L; Servenius, B; ... Peterson, PA; + view all (1982) Isolation and identification of a cDNA clone coding for an HLA-DR transplantation antigen alpha-chain. Scand J Immunol , 16 (4) pp. 303-308.

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Abstract

Membrane-bound mRNA was isolated from Raji cells and enriched for message coding for the HLA-DR transplantation antigen alpha-chain by sucrose gradient centrifugation. Double-stranded cDNA was constructed from this mRNA fraction, ligated to plasmid pBR322, and cloned into Escherichia coli. By hybrid selection, a plasmid, pDR-alpha-1, able to hybridize with mRNA coding for the HLA-DR alpha-chain was identified. From the nucleotide sequence of one end of the insert an amino acid sequence was predicted which is identical to part of the amino-terminal sequence of an HLA-DR alpha-chain preparation isolated from Raji cells. This clearly shows that pDR-alpha-1 carries almost the complete message for an HLD-DR alpha-chain. From the nucleotide sequence of this plasmid it will be possible to predict the primary structure of an HLA-DR alpha-chain.

Type: Article
Title: Isolation and identification of a cDNA clone coding for an HLA-DR transplantation antigen alpha-chain.
Location: England
Keywords: Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Genes, Genes, MHC Class II, HLA-DR Antigens, Humans, Plasmids
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/1354945
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