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Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.

Wiman, K; Larhammar, D; Claesson, L; Gustafsson, K; Schenning, L; Bill, P; Böhme, J; ... Rask, L; + view all (1982) Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain. Proc Natl Acad Sci U S A , 79 (6) pp. 1703-1707. 10.1073/pnas.79.6.1703.

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Abstract

The HLA-D locus in the major histocompatibility complex controls the expression of the genetically polymorphic HLA-DR antigens. mRNA coding for the beta chains of these antigens was partially purified from the human lymphoblastoid cell line Raji. The mRNA was copied into double-stranded cDNA and cloned in Escherichia coli. One clone, pDR-beta-1, obtained by hybrid selection, carries a 1070-base-pair insert comprising all of the coding region except the signal sequence and a substantial portion of the untranslated region. To identify pDR-beta-1, highly purified HLA-DR antigen beta chains derived from Raji cells were subjected to NH2-terminal amino acid sequence determination. This sequence displayed extensive homology with that deduced from the nucleotide sequence at the 5' end of the pDR-beta-1 coding region. Taken together, the amino acid and nucleotide sequences strongly argue in favor of Raji cells containing at least two beta-chain loci.

Type: Article
Title: Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.
Location: United States
DOI: 10.1073/pnas.79.6.1703
Keywords: Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, DNA, HLA-DR Antigens, Histocompatibility Antigens Class II, Humans, Macromolecular Substances, Plasmids, RNA, Messenger
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/1354944
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