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Adoptive therapy with redirected primary regulatory T cells results in antigen-specific suppression of arthritis

Wright, GP; Notley, CA; Xue, SA; Bendle, GM; Holler, A; Schumacher, TN; Ehrenstein, MR; (2009) Adoptive therapy with redirected primary regulatory T cells results in antigen-specific suppression of arthritis. P NATL ACAD SCI USA , 106 (45) 19078 - 19083. 10.1073/pnas.0907396106.

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Abstract

Regulatory T cells (Tregs) can suppress a wide range of immune cells, making them an ideal candidate for the treatment of auto-immunity. The potential clinical translation of targeted therapy with antigen-specific Tregs is hampered by the difficulties of isolating rare specificities from the natural polyclonal T cell repertoire. Moreover, the initiating antigen is often unknown in autoimmune disease. Here we tested the ability of antigen-specific Tregs generated by retroviral gene transfer to ameliorate arthritis through linked suppression and therefore without cognate recognition of the disease-initiating antigen. We explored two distinct strategies: T cell receptor (TCR) gene transfer into purified CD4+CD25+ T cells was used to redirect the specificity of naturally occurring Tregs; and co-transfer of FoxP3 and TCR genes served to convert conventional CD4(+) T cells into antigen-specific regulators. Following adoptive transfer into recipient mice, the gene-modified T cells engrafted efficiently and retained TCR and FoxP3 expression. Using an established arthritis model, we demonstrate antigen-driven accumulation of the gene modified T cells at the site of joint inflammation, which resulted in a local reduction in the number of inflammatory Th17 cells and a significant decrease in arthritic bone destruction. Together, we describe a robust strategy to rapidly generate antigen-specific regulatory T cells capable of highly targeted inhibition of tissue damage in the absence of systemic immune suppression. This opens the possibility to target Tregs to tissuespecific antigens for the treatment of autoimmune tissue damage without the knowledge of the disease-causing autoantigens recognized by pathogenic T cells.

Type: Article
Title: Adoptive therapy with redirected primary regulatory T cells results in antigen-specific suppression of arthritis
DOI: 10.1073/pnas.0907396106
Keywords: autoimmunity, gene therapy, T-cell receptor, IMMUNOLOGICAL SELF-TOLERANCE, TGF-BETA, AUTOIMMUNE-DISEASE, CUTTING EDGE, FOXP3, MICE, DIFFERENTIATION, INTERLEUKIN-17, LYMPHOCYTES, EXPRESSION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/135411
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