Hurtado-López, P;
Murdan, S;
(2006)
An investigation into the adjuvanticity and immunogenicity of zein microspheres being researched as drug and vaccine carriers.
J Pharm Pharmacol
, 58
(6)
769 - 774.
10.1211/jpp.58.6.0007.
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Abstract
We have determined whether zein microspheres could act as vaccine adjuvants i.e. increase the immune responses to co-administered immunogens. Ovalbumin (model antigen)-loaded zein microspheres, blank zein microspheres and ovalbumin solution were intramuscularly administered to mice and the sera antibody levels were determined by ELISA. Another group of mice was orally dosed with blank zein microspheres, and serum and faecal antibody levels were determined. As expected, negligible antibody titres were obtained with the ovalbumin solution. Surprisingly, intramuscular administrations of blank zein microspheres elicited high levels of serum IgG which bound to the ovalbumin antigen coated on ELISA microtitre plates. This indicated that anti-zein antibodies had been elicited by blank zein microspheres and that these antibodies were cross-reacting with ovalbumin antigen coated onto ELISA plates. Such cross-reactivity inhibited the determination of the adjuvant activity of zein microspheres, if any. Additional ELISA assays, where zein was used as the coating antigen, confirmed the generation of anti-zein antibodies by blank zein microspheres i.e. zein microspheres were immunogenic following intramuscular administration. Upon oral administration of blank zein microspheres, serum IgG levels remained low but intestinal IgA levels increased following booster doses i.e. systemic tolerance, but not mucosal tolerance, to oral zein particles was achieved. Zein microspheres were immunogenic when administered intramuscularly and orally.
| Type: | Article |
|---|---|
| Title: | An investigation into the adjuvanticity and immunogenicity of zein microspheres being researched as drug and vaccine carriers. |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1211/jpp.58.6.0007 |
| Publisher version: | http://dx.doi.org/10.1211/jpp.58.6.0007 |
| Language: | English |
| Additional information: | This is the pre-peer-reviewed version of the following article: Hurtado-López, P. and Murdan, S. (2006), An investigation into the adjuvanticity and immunogenicity of zein microspheres being researched as drug and vaccine carriers. Journal of Pharmacy and Pharmacology, 58: 769–774. doi: 10.1211/jpp.58.6.0007, which has been published in final form http://onlinelibrary.wiley.com/doi/10.1211/jpp.58.6.0007/abstract |
| Keywords: | Administration, Oral, Animals, Drug Carriers, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulin A, Immunoglobulin G, Injections, Intramuscular, Mice, Mice, Inbred BALB C, Microspheres, Vaccines, Zein |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1351465 |
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