Verhoekx, JSN; van der Werff, JFA; Mudera, V; (2011) Cytokines modulated myofibroblast induction and force generation in dupuytren's fibroblasts; Lessons for tissue engineering? In: Abstracts of the Tissue and Cell Engineering Society (TCES) meeting 19-21 July 2011, University of Leeds. (pp. 45 - 45).

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Abstract

Tissue engineering aims to get cells in vitro to lay down collagen and generate living functional 3D structures for implantation. The major challenge is getting fibroblasts to lay down functional collagen in vitro. We studied a natural condition where fibroblasts secrete excess collagen and shorten the matrix by remodelling it (Dupuytren’s disease). Some of the collagen layed down is organised and has the structure of tendon (cord) but the active state is the nodule. The cells responsible for the fibrotic contractures seen in Dupuytren’s disease are the myofibroblasts, expressing α-smooth muscle actin (α-SMA), correlating with increased deposition of extracellular matrix (ECM) components1 and contractile force generation, contracting the ECM2. Cytokines are potential targets for myofibroblast modulation, as they control expression of myofibroblast marker α-SMA3. We have examined the effect of cytokines, TGF-β1 and PDGF-BB, on force generation and matrix remodeling.

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