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Protein Adsorption on Nanocrystalline TiO(2) Films:  An Immobilization Strategy for Bioanalytical Devices.

Topoglidis, E; Cass, AE; Gilardi, G; Sadeghi, S; Beaumont, N; Durrant, JR; (1998) Protein Adsorption on Nanocrystalline TiO(2) Films:  An Immobilization Strategy for Bioanalytical Devices. Anal Chem , 70 (23) 5111 - 5113. 10.1021/ac980764l.

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Abstract

We have investigated the use of optically transparent, nanoporous TiO(2) films as substrates for protein immobilization. Immobilization on such films may be readily achieved from aqueous solutions at 4 °C. The nanoporous structure of the film greatly enhances the active surface area available for protein binding (by a factor of 150 for a 4-μm-thick film). We demonstrate that the redox state of immobilized cytochrome c may be modulated by the application of an electrical bias potential to the TiO(2) film and that the fluorescence yield of immobilized fluorophore-labeled maltose-binding protein may be used to monitor specifically maltose concentration. We conclude that nanoporous TiO(2) films may be useful both for basic studies of protein/electrode interactions and for the development of array-based bioanalytical devices employing both optical and electrochemical signal transduction methodologies.

Type:Article
Title:Protein Adsorption on Nanocrystalline TiO(2) Films:  An Immobilization Strategy for Bioanalytical Devices.
Location:United States
DOI:10.1021/ac980764l
Language:English

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