Evidence for the presence of a reversible Ca2+-dependent pore activated by oxidative stress in heart mitochondria.
Rat heart mitochondria became permeabilized to sucrose when incubated with 100 nmol of Ca2+/mg of protein in the presence of Pi. Ca2+ chelation with EGTA restored impermeability to sucrose, which became entrapped in the matrix space. t-Butylhydroperoxide markedly promoted permeabilization in the presence of Ca2+ but not in its absence, and Ca2+-plus-t-butylhydroperoxide-induced permeabilization was reversed by EGTA. The data suggest that Ca2+ and oxidative stress synergistically promote the reversible opening of an inner membrane pore.
|Title:||Evidence for the presence of a reversible Ca2+-dependent pore activated by oxidative stress in heart mitochondria.|
|Keywords:||Animals, Calcium, Egtazic Acid, Indicators and Reagents, Intracellular Membranes, Male, Mitochondria, Heart, Onium Compounds, Organophosphorus Compounds, Oxygen, Permeability, Peroxides, Rats, Rats, Inbred Strains, tert-Butylhydroperoxide|
|UCL classification:||UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
Archive Staff Only