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The sequestration of Ca by mitochondria in rat heart cells

Kessar, P; Crompton, M; (1983) The sequestration of Ca by mitochondria in rat heart cells. Cell Calcium , 4 (4) 295 - 305. 10.1016/0143-4160(83)90006-4.

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Abstract

Rat heart ventricular cells, purified by Percoll density gradient centrifugation, were incubated in the presence of 1.3 mM CaCl. After 20 min incubation, samples of the cells were lysed in medium containing 0.3 mM digitonin, ruthenium red and EGTA, and a mitochondrial fraction was isolated at intervals thereafter. Extrapolation of the mitochondrial Ca contents to zero time enabled the endogenous Ca to be estimated at the time of cell lysis. The lysis conditions yielded essentially complete release of lactate dehydrogenase from the cells, but caused negligible damage to the mitochondria as judged by their retention of glutamate dehydrogenase, and their ability to accumulate and retain Ca in the absence of ruthenium red and EGTA. The data indicate that about 13% of total cell CA only may be mitochondrial in vivo. © 1983.

Type: Article
Title: The sequestration of Ca by mitochondria in rat heart cells
DOI: 10.1016/0143-4160(83)90006-4
UCL classification: UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)
URI: http://discovery.ucl.ac.uk/id/eprint/1344259
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